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Part:BBa_K4171014
PT7-tRNA(Ser)
Background
This is the DNA sequence of native tRNASer (BBa_K4171007) of E.coli , which can bind with serine (Ser) by the help of enzyme SerRS (BBa_K4171001). With T7 promoter sequence (BBa_I719005) in the front, RNA polymerase is able to work properly on the template when doing in vitro RNA synthesis.
![](https://static.igem.wiki/teams/4171/wiki/parts/in-vitro-pathway.png)
Fig. 1. In vitro Sec synthesis pathway
Usage
This sequence will be synthesized through in vitro RNA synthesis, which produces tRNASer transcript, the substrate of SerRS for seryl-tRNA aminoacylation in tRNA selenocysteine (Sec) synthesis pathway.
Characterization
![](https://static.igem.wiki/teams/4171/wiki/parts/pt7-ser.png)
Fig. 2. Confirmation of PT7 -tRNASer (BBa_K4171014) constructed through PCR. M: Marker; Lane1: PT7 -tRNASer (111 bp)
Reference
[1]Asahara, H., Himeno, H., Tamura, K., Nameki, N., Hasegawa, T., & Shimizu, M. (1994). Escherichia coli SERYL-trna synthetase recognizes tRNASer by its characteristics tertiary structure. Journal of Molecular Biology, 236(3), 738–748. [2]https://parts.igem.org/Part:BBa_I719005
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 58
- 1000COMPATIBLE WITH RFC[1000]
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